The paper presents an analysis of patterns of punctuation in four manuscript versions of the Annunciation narrative (Luke 1:26–38) dating to the fourteenth and fifteenth centuries and in four printed translations of the sixteenth and seventeenth centuries, looking at the repertoire of the forms of punctuation available and at their employment. There is no continuation between the period of manuscript and print. The density of punctuation varies considerably in the manuscripts, and the print versions generally employ more punctuation than the manuscripts. A trend in the print versions can be observed for a consolidation of the inventory of punctuation symbols. In the period under discussion, some fuzziness and variation remain with regard to their use, particularly of the colon and of the formats for the marking of direct speech. This small-scale test case is intended to indicate the potential of researching patterns of (ir)regularities underlying the distribution of punctuation marks.